22株乳杆菌的自聚和粘附特性研究.doc

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 Aggregation and adhesion properties of 22 Lactobacillus strains# 10 15 20 25 30 35 40 45  Abstract: In this paper, the auto-aggregating, co-aggregating, hydrophobicity and adhering abilities of 22 Lactobacillus strains belonging to different species were assessed. There was no correlation between auto-aggregation and adhesion of the strains belonging to different species, while there was positive correlation (P0.05) between auto-aggregation and adhesion of the strains belonging to same species. After treating by guanidine HCl, the auto-aggregating and adhering abilities of some Lactobacillus strains decreased, indicating that surface-bound protein and other macro-molecular played role in the adhering and auto-aggregating abilities. The strains Lactobacillus plantarum 20 and 66 had higher adhdsion and coaggregation abilities should be further studied for their probably probiotic properties. Aggregating, co-aggregating and adhering abilities of Lactobacillus strains could be just used as the preliminary criteria for selecting strains having probiotic potential. Key words: auto-aggregating, coaggregating, hydrophobicity, adhesion 0 Introduction A probiotic is generally defined as “a live microorganism which, when administered in adequate amounts, confers health benefit on the host” [1]. Lactobacillus could exert beneficial effect on humans by inhibiting invasion of pathogens, improving the epithelial barrier function, modulating host immune system, and so on [2]. Many strains of Lactobacilli have a variety of health-promoting effects in humans. Adherence and colonization of Lactobacillus strains in gastrointestinal tract is the prerequisite for the strains to exhibit beneficial effect on humans[3]. The assessment of autoaggregation, coaggregation, hydrophobicity and adherence to intestinal epithelial cell lines (Caco-2 cell and HT-29 cell) of Lactobacillus strains has been used as in vitro methods to screen strains having probiotic potential[4-7]. Human enteroc

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