基于GCMS 对1 株芽孢杆菌FJAT-13831 的鉴定.docVIP

基于GC/MS对1株芽孢杆菌FJAT-13831的鉴定 刘国红1,2，刘波1(，林乃铨2，陈峥1 (1福建省农业科学院农业生物资源研究所福州 350003；2福建农林大学福州350002) 摘要：GC/MS检测芽孢杆菌分离菌株FJAT-13831胞外物质成分，进行主成分和聚类分析不同的芽孢杆菌种间具有显著性差异，FJAT-13831该方法可从代谢水平上对芽孢杆菌进行，为芽孢杆菌的提供一种新的技术手段关键词：分类鉴定；代谢组学；芽孢杆菌；胞外物质； of Bacillus species FJAT-13831 based on the gas chromatography/mass spectrometer LIU Guo-Hong1,2, LIU Bo1(, LIN Nai-Quan2, CHEN Zheng1 (1. Agricultural Bioresources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 2. Fujian Agriculture and Forestry University, Fuhzou 350002, China) ABSTRACT: Objective Since the genetic relationship of several Bacillus species were very close, it is difficult to distinguish them by the usual classification methods. To rapidly identify the Bacillus species with close relatives, Bacillus metabolomics was studied in this paper. Method Based on Gas Chromatography-Mass Spectrometer (GC/MS) detection, principal component and cluster analysis were performed on extracellular substance of 10 Bacillus species. Results It was showed that the isolate FJAT-13831 may be a new species of the genus Bacillus, and different Bacillus species had significant differences. Conclusion The method used in the paper may be identified Bacillus species with close relatives at the metabolic level, and provided a new technical means for the Bacillus classification and identification. KEY WORDS: Classification and identification; metabolomics; genus Bacillus; extracellular substance 1 引 言 芽孢杆菌是一类革兰氏阳性细菌，。由于种间相似性很高，常用的分类方法很难将极准确区分开来，这就需要一种新的方法来对这些进行分类鉴定。 随着生命科学的发展，从分子水平研究生命现象的技术手段不断产生，其中代谢组学是20世纪90年代后期产生的新兴学科。代谢组学(etabolomics)是一门对某一生物或细胞所有低相对分子质量代谢产物进行定性和定量分析的学科，具有中体动态、无偏向性、研究技术多样、实验方法灵活等特点，其流程主要包括代谢谱差异分析、潜在生物标记物鉴定及代谢通路分析。与传统依赖单一标志物的细菌鉴别方法相比，代谢组学揭示的是系列关联生物标记物的综合差异，因此具有更高的准确性。近年来代谢组学逐步成为研究热点，被人们广泛应用在各个领域。 关于GC/MS检测芽孢杆菌胞外物质成分的文献在国内还未见报道 本实验室从土壤中分离到一株芽孢杆菌FJAT-13831，根据16S rRNA序列相似性分析发现，与蜡状芽孢杆菌类群相似皆达99%以上，但其表型特征差异很大[6]。故本利用GC/MS对的物质成分进行检测，通过分析种间物质成分差异未知菌株 2 材料与方法 .1 供试菌株 Bacillus marisflavi DSM 16204T，Bacil