酶解带鱼蛋白亚铁螯合多肽抗氧化性研究【毕业论文】.doc

本科毕业论文 （20 届） 酶解带鱼蛋白亚铁螯合多肽抗氧化性研究 专业：食品科学与工程 目录 摘 要 1 Abstract 2 1 前言 3 2 实验材料、主要仪器与试剂 3 2.1实验材料 3 2.2主要仪器 3 2.3主要试剂 3 3 实验方法 4 3.1酶解及亚铁螯合工艺流程 4 3.2羟自由基清除活性的测定 4 3.3超氧阴离子自由基清除活性的测定 5 3.4 DPPH自由基清除活性的测定 5 4 结果与分析 5 4.1对羟自由基的清除作用 5 4.2对超氧阴离子自由基的清除作用 6 4.3对DPPH自由基的清除作用 7 5 小结 8 5.1带鱼酶解蛋白亚铁螯合多肽具有清除自由基的能力 8 5.2未螯合酶解蛋白样液清除自由基能力很弱 8 参考文献 9 致谢 11 附录Ⅰ文······················································································12 附录文······················································································25  [摘要] 用舟山带鱼下脚料为原料，利用碱性蛋白酶，木瓜蛋白酶和胰蛋白酶水解制备多肽亚铁螯合物，并对亚铁鳌合物抗氧化活性及自由基活性进行了初步研究。本文从清除羟基自由基(·OH)、超氧阴离子(O2-·)和1, 1-二苯基-2-苦苯肼自由基(DPPH·)三个方面，探讨纯化方法对亚铁鳌合物清除自由基活性的影响。以抗坏血酸为对照，利用分光光度法分别测定其清除自由基活性能力。结果显示：三种带鱼酶解蛋白螯合水解物的浓度越高，其清除自由基活性也越强，但在相同浓度范围内，Vc的清除率远远大于三种带鱼酶解蛋白螯合水解物，未螯合的酶解蛋白样液没有明显的自由基清除作用。 [关键词] 带鱼下脚料；螯合物；抗氧化；清除；自由基 Study the antioxidation on compound enzymic peptides iron from the enzymolysis hairtail [Abstract] With the hairtail off-cuts as material, as original material,and use the hydrolyze of trypsin, papain, alcalase to make compound enzymic peptides iron,and give a original research of the activity of the antioxidation of compound enzymic peptides iron and radical reactivity.From the dumping of three aspects of ·OH, O2-·,DPPH·to study the influence of the way of purofication did. According to the ascrobic acid,and measure the radical activity through the method of spectrophotometry. The result shows: the high density of the three kinds of the enzymolysis of compound enzymic peptides,the high ability of dumping the material`s radical activity. But at the same density ,the dumping rate of vc is far better than the three kinds of the enzymolysis of compound enzymic peptides,and the uncompound enzymic peptides protain sample liquid has less obvious ability to clean up the radical reactivity. [Key words] Hairtail off-cuts;Chelete substance;Antioxidation;Dumping; Radical reactivity 1 前言 带鱼又叫刀鱼、牙带鱼，是鱼纲鲈形目带鱼科动物是著名的一大海洋鱼