冀产远志与卵叶远志的含量测定研究.docVIP

冀产远志与卵叶远志的含量测定研究 　　[摘要] 目的 探讨冀产远志与卵叶远志的种间差异，以指导临床合理用药。 方法 采用高效液相色谱法测定冀产远志与卵叶远志在不同采集期、不同用药部位中有效活性成分远志酮Ⅲ与3，6二芥子酰基蔗糖的含量。 色谱柱为Sunfire C18（4.6 mm×250 mm，5 μm），流动相为甲醇-0.05%磷酸溶液（70∶30），检测波长为230 nm，流速为1.0 mL/min，柱温30℃。 结果 各组分分离度良好，远志口山酮Ⅲ在0.46003～4.6003 mg/mL浓度范围内与峰面积线性关系良好（r=0.999）；3，6二芥子酰基蔗糖在0.41587～4.15872 mg/mL浓度范围内与峰面积线性关系良好（r=0.999），平均回收率分别为98.10%（RSD=1.80%）和98.13%（RSD=1.63%），7批样品中远志酮Ⅲ的含量分别为0.113、0.078、0.121、0.105、0.082、0.054、-0.003 g/g，3，6二芥子酰基蔗糖的含量为0.665、0.392、0.390、0.453、0.394、0.586、0.411 g/g。 结论 该方法简便、快速，为冀产远志的品种开发提供科学数据。 　　[关键词] 远志； 卵叶远志；含量测定；高效液相色谱法 　　[中图分类号] R927.2 [文献标识码] A [文章编号] 1673-7210（2016）04（c）-0132-04 　　[Abstract] Objective To discuss the difference between Polygala tenuifolia Willd. and Polygala sibirica L. for guiding clinical rational drug use. Methods Effective active ingredient Polygalaxanthone and 3，6'-Disinapoylsucrose content of different collection period and parts of Polygala tenuifolia Willd and Polygala sibirica L were measured by HPLC method. The determination was performed on Sunfire C18 （4.6 mm×250 mm， 5 μm） column with methanol -0.05% phosphate acid （70∶30） as mobile phase at detection wavelength of 230 nm， the flow rate was 1.0 mL/min， the column temperature was set at 30℃. Results The components were separated completely. The good linear relationship of Polygalaxanthone Ⅲ occurred in the range of 0.460 03～4.6003 mg/mL（r = 0.999）， the good linear relationship of 3，6'-Disinapoylsucrose in the range of 0.415 87～4.158 72 mg/mL（r = 0.999）. The average recoveries were 98.10% （RSD=1.80%） and 98.13% （RSD=1.63%）. In the 7 groups， the content of Polygalaxanthone Ⅲ was 0.113， 0.078， 0.121， 0.105， 0.082， 0.054， -0.003 g/g， 3，6'-Disinapoylsucrose was 0.665， 0.392， 0.390， 0.453， 0.394， 0.586， 0.411 g/g. Conclusion The method is simple and quick， which can be used for rational development of Polygala tenuifolia Willd. 　　[Key words] Polygala tenuifolia Willd.； Polygala sibirica L.； Content determination；HPLC method 　　远志为远志科植物远志（Polygala tenu