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sodiumchloride.PDF
Mitomycin C
from Streptomyces caespitosus
Catalog Number M0503 and M4287
Storage Temperature 2–8 °C
CAS RN 50-07-7 Mitomycin C inhibits DNA synthesis. It reacts covalently
Synonyms: Ametycine; Mutamycin; MMC; Mitocin-C with DNA, in vivo and in vitro, forming crosslinks
between the complementary strands of DNA. This
Product Description interaction prevents the separation of the
complementary DNA strands, thus inhibiting DNA
replication. Mitomycin C is one of the few antibiotics
known to interact with DNA in this fashion.6,7
These products are supplied in vials containing 50 mg
of powder, of which 2 mg is Mitomycin C and 48 mg is
sodium chloride.
Catalog Number M4287 is cell culture tested and the
recommended concentration for eukaryote cell culture
use is 10–50 mg/mL.8
Molecular formula: C15H18N4O5 Preparation Instructions
Molecular Weight: 334.33 Mitomycin C is soluble in water (0.5 mg/mL).
1
pKa values: 3.2, 6.5
A 0.5% solution in water has a pH of 6.0–7.5.9 A
EmM(367 nm) = 21.84 (water) saturated solution has a pH of 5.0–9.0.3
EmM(357 nm) = 22.78 (methanol)1
E1%(216 nm) = 742 (methanol) Mitomycin C undergoes rapid degradation in acidic
1%
E (360 nm) = 742 (methanol) solutions (pH 6.0).10 It is mos
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