AtHKT1;1 Mediates Nernstian Sodium Channel Transport Properties in Arabidopsis Root Stelar Cells 英文参考文献.docVIP
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AtHKT1;1 Mediates Nernstian Sodium Channel Transport Properties in Arabidopsis Root Stelar Cells 英文参考文献
AtHKT1;1MediatesNernstianSodiumChannelTransport
PropertiesinArabidopsisRootStelarCells
ShaowuXue1¤a,XuanYao1¤b,WeiLuo1,3,DeepaJha2,MarkTester2,TomoakiHorie1¤c,JulianI.
Schroeder1*
1DivisionofBiologicalSciences,CellandDevelopmentalBiologySection,UniversityofCalifornia,SanDiego,LaJolla,California,UnitedStatesofAmerica,2Australian
CentreforPlantFunctionalGenomics,UniversityofAdelaide,SouthAustralia,Australia,3StateKeyLaboratoryofCropGeneticsandGermplasmEnhancement,Collegeof
ResourcesandEnvironmentalSciences,NanjingAgriculturalUniversity,Nanjing,China
Abstract
TheArabidopsisAtHKT1;1proteinwasidentifiedasasodium(Na+)transporterbyheterologousexpressioninXenopuslaevis
oocytes and Saccharomyces cerevisiae. However, direct comparative in vivo electrophysiological analyses of a plant HKT
transporterinwild-typeandhktloss-of-functionmutantshasnotyetbeenreportedandithasbeenrecentlyarguedthat
heterologousexpressionsystemsmayalterpropertiesofplanttransporters,includingHKTtransporters.Inthisreport,we
analyze several key functions of AtHKT1;1-mediated ion currents in their native root stelar cells, including Na+ and K+
conductances,AtHKT1;1-mediatedoutwardcurrents,andshiftsinreversalpotentialsinthepresenceofdefinedintracellular
and extracellular salt concentrations. Enhancer trap Arabidopsis plants with GFP-labeled root stelar cells were used to
investigate AtHKT1;1-dependent ion transport properties using patch clamp electrophysiology in wild-type and athkt1;1
mutantplants.AtHKT1;1-dependentcurrentswerecarriedbysodiumionsandthesecurrentswerenotobservedinathkt1;1
+
mutant stelar cells. However, K currents in wild-type and athkt1;1 root stelar cell protoplasts were indistinguishable
correlatingwiththeNa overK selectivityofAtHKT1;1-mediatedtransport.Moreover,AtHKT1;1-mediatedcurrentsdidnot
+
+
+
show a strong voltage dependence in vivo. Unexpectedly, removal of extracellular Na caused a reduction in AtHKT1;1-
mediatedoutwardcurrentsinColumbiarootstelarcellsandXenopusoocytes,indic
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