36.盐炙对车前子中京尼平苷酸、毛蕊花糖苷和异毛蕊花糖苷含量的影响----卢丹逸.pdf

盐炙对车前子中京尼平苷酸、毛蕊花糖苷和异毛蕊花糖苷含量的影响 卢丹逸，黄婉茹，马志国* 暨南大学 药学院，广东 广州 510632 *通讯作者：马志国，副教授，从事中药炮制学与中药药剂学的教学和科研工作。 Tel:(020E-mail:mzg79@ 摘 要：目的：建立车前子中京尼平苷酸、毛蕊花糖苷和异毛蕊花糖苷的RP-HPLC 含量测定方法，并研究三个成分在盐炙前后的含量变化情况；方法：采用Phecda ODS(150 mm × 4.6 mm ，5 μm)色谱柱，柱温：30℃；流动相为甲醇（A ）-0.1% 甲酸（B ），梯度洗脱（0 min，15% A；5 min，20% A ；30 min，55% A ）,流速： 1.0 mL/min；检测波长：250 nm 和330 nm 。比较7 批车前子盐炙前后京尼平苷 酸、毛蕊花糖苷和异毛蕊花糖苷的含量。结果：方法学考察结果表明，所建立的 HPLC 方法准确、稳定、可靠；车前子中的京尼平苷酸、毛蕊花糖苷炮制后含量 降低，异毛蕊花糖苷盐炙后含量增加。结论：车前子中毛蕊花糖苷在炮制过程中 可能部分转化为异毛蕊花糖苷。将京尼平苷酸、毛蕊花糖苷和异毛蕊花糖苷共同 作为评价车前子及其炮制品的质量控制的指标成分更加合理。 关键词：车前子；盐炙；京尼平苷酸；毛蕊花糖苷；异毛蕊花糖苷 Reaserch on the main components in Semen Plantaginis about processing * LU Danyi ，HUANG Wanru ，MA Zhi-guo College of Pharmacy, Jinan University, Guangzhou 510632, China; Abstract: Objective: To establish an RP-HPLC method for determination of Geniposidic acid, Acteoside and Isoacteoside in Semen Plantaginis pieces, and the three components in the change situation, stir fried with salt. Method: the Phecda ODS (150 mm × 4.6 mm, 5 μ m) column, the column temperature: 30 ℃; the mobile phase of methanol (A) -0.1% acid (B), Gradient elution (0 min, 5 min, 15% A; 20% A; 30 min, 55% A), flow rate: 1 mL/min; the detection wavelength: 250 nm and 330 nm. Comparison of different batches of plantain seed processing fingerprint determination of the contents of Geniposidic acid, Acteoside and Isoacteoside and psyllium Plantago in pieces and salt. Results: The study results show that the HPLC method, the method is accurate, stable, reliable, Geniposidic acid, Acteoside content decreased after processing after processing, isoacteoside content increased. Conclusion: Acteoside in Plantago may be partially converted into isoacteoside in the processing process, Geniposidic acid, acteoside and isoacteoside as the commo