芒鲶胶原组织再生膜的制备及骨修复作用研究-中国海洋药物.doc

芒鲶胶原组织再生膜的制备及骨修复作用研究 夏凯，吴文惠1*, 易杨华2* 上海海洋大学 食品学院，上海，201306； 2.中国人民解放军第二军医大学 海洋药物研究中心，上海，200433） 摘 要 :目的 研究芒鲶胶原组织再生膜（Paugusiushamiltoa collagen tissue regeneration membrane,简称PCM )的制备、物理性质、生物学特性以及引导骨组织再生的特性。方法 利用芒鲶鱼皮胶原蛋白为原料，采用脱色、脱脂、脱细胞和热交联方法制备组织再生膜。通过电镜扫描（SEM）、吸水率、孔隙率和力学强度评价其理化特性。通过兔颅骨骨缺损模型，评价PCM引导骨组织再生的效果。结果 SEM扫描结果显示，PCM呈双层膜结构，一侧为网状多孔结构，另一侧光滑致密；其吸水率约为自身质量的4倍，孔隙率为78%，平均拉伸应力最大值为18.82 MPa；皮内刺激反应显示PCM未造成红斑、焦痂形成和水肿，对皮肤无刺激性；以瑞士Bio-Gide 膜作为阳性对照组，在4周和8周后，通过显微CT、苏木精-伊红染色（HE染色），兔颅骨骨缺损修复动物实验表明，PCM能屏障纤维结缔组织，诱导成骨细胞生长，具有引导骨再生的作用。结论 PCM具有优良的生物材料特性 关键词：芒鲶；胶原蛋白；组织再生膜；生物医用材料 中图分类号：R965 Preparation of tissue regeneration collagen membrane from Paugusiushamiltoa and research for bone repairing effect XIA Kai12, CHEN Tong2, WU Wen-hui1*, YI Yang-hua2* College Of Food Science Technology ,Shanghai Ocean University, Shanghai 201306, China; 2 Research Center For Marine Drugs, Second Military Medical University, Shanghai 200433, China) Abstract: Objective Tissue regenerating collagen membrane was prepared Paugusiushamiltoa and the physical and biological characteristics was evaluated for bone tissue regeneration. Methods The bone tissue regenerating membrane was derived from Paugusiushamiltoa and prepared by decolorization， and cross-linking. The physical and biological properties were evaluated with microstructure，porosity , water absorption, mechanical and degradation properties. The effect of bone repair were evaluated by calvarial defect models of New Zealand rabbit. Results The bilayer structure including a dense layer and a loose layer of the surface morphology of PCM was revealed by SEM. The porosity rate, water absorption rate and average tensile stress were 78%, 4 times than itself and 18.82 MPa respectively. Intradermic reaction test showed that PCM had no skin irritation. The repairing effects were presented by Micro-CT and the image analysis of the hematoxylin-eosin staining ( HE ) of calvarial defect models with PCM, Bio-Gide membrane as positive control after 4 and 8 w