金银花DNA去甲基化酶基因的生物信息学分析_齐琳洁.pdf

Acta Pharmaceutica Sinica 2015, 50 (3): 367−371 367 DNA 1, 2 2* 2 2 1 , , , , (1. , 430023; 2. , , 100700) : DNA , 4 DNA , LJDME1LJDME2LJDME3 LJDME4 , LJDME1LJDME2 , LJDME3LJDME4 , DME , 4 DNA , LJDME1LJDME2 , , 4 DNA DNA : ; ; : R931 : A : 0513-4870 (2015) 03-0367-05 Bioinformatics analysis of DNA demethylase genes in Lonicera japonica Thunb 1, 2 2* 2 2 1 QI Lin-jie , YUAN Yuan , WU Chong , HUANG Lu-qi , CHEN Ping (1. Wuhan Polytechnic University, Wuhan 430023, China; 2. State Key laboratory of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China) Abstract : The DNA demethylase genes are widespread in plants. Four DNA demethylase genes (LJDME1 , LJDME2 , LJDME3 and LJDME4) were obtained from transcriptome dataset of Lonicera japonica Thunb by using bioinformatics methods and the proteins physicochemical properties they encoded were predicted. The phylogenetic tree showed that the four DNA demethylase genes and Arabidopsis thaliana DME had a close relationship. The result of gene expression model showed that four DNA demethylase genes were different between species. The expression levels of LJDME1 and LJDME2 were even more higher in Lonicera japonica var. chinensis than t