丹参联合胞二磷胆碱治疗大鼠脑挫伤机理探究.doc

丹参联合胞二磷胆碱治疗大鼠脑挫伤机理探究 　【摘要】 目的：探讨丹参与胞二磷胆碱联合应用治疗脑损伤的机制。方法:取成年wistar大鼠，以自由落体法致大鼠脑挫裂伤，采用721分光光度计检测伤后24h、7d和14d时，治疗组与对照组脑组织中SOD、MDA、GSHPX和Ca2+含量。结果：治疗组SOD和GSHPX活性提高，而且与模型组及对照组比较，SOD有显著性差异（Plt;005）。治疗组脑组织Ca2+含量明显降低。结论:丹参在治疗脑损伤时，可以提高SOD活性，拮抗自由基和钙离子超载对脑的损伤。 　　【关键词】 丹参胞二磷胆碱；大鼠；脑挫伤 　　【ABSTRACT】 Objective: To investigate the morphological changes of brain contusion treated by Salvia miltiorrhiza.methods:The Feeneys falling body injury method was used to make 45 wistar rats into brain contusion model. Then the rats were randomly divided into two groups, i.e. experimental group including 30 animals were treated with Salvia miltiorrhiza and Cytidine diphosphale choline through abdominal injection,15rats without any treatment were taken as control group. After 2d,14d, and 28d of brain contusion, the morphological change was observed at the injured area by LM and EM and the contents of SOD, MDA in serum were observed.Results:The morphological observation revealed that the lesioned neuron restored faster and scar tissue formated early in the experimental group. The concentration of SOD in serum was significantly higher in the experimental group than that in the control group (Plt;005). the concentration of MDA was lower in the experimental group than that in the control group.Conclusion: It might promote the restoration of brain contusion to treat the rats with Salvia miltiorrhiza and Cytidine diphosphale choline. 　　【KEY WORDS 】 Brain contusion;Salvia miltiorrhiza;Rat 脑损伤区由于缺血、缺氧导致神经元和胶质细胞的损伤，在早期损伤呈进行性加重，至3~7d后才被控制［1,2］，为了及早改善机体微循环，在脑挫伤早期是否应用丹参液，是值得探讨的。有人在脑损伤早期应用丹参注射液收到了良好的效果［4,5］；为了探讨脑挫伤应用丹参的机理，我们复制大鼠脑挫伤模型，在伤后连续注射丹参液，除观察伤灶区变化外，还检测了脑中钙离子（Ca2+）、超氧化物歧化酶（SOD）、丙二醛（MDA）和谷光甘肽过氧化物酶（GSHPX）含量，将治疗组与模型组进行了比较，以阐明丹参作用的可能机理。 　　1 材料和方法 　　取wistar大鼠72只，体重180~250g，雌雄不拘，分笼喂养。随机分为正常对照组、模型组和治疗组。采用Feeney氏自由落体法复制脑挫伤模型。治疗组于伤后即可肌注丹参液（10/kg）和胞二磷胆碱钠注射液（225mg/kg），2次/d。于术后24h和7d，分别断头处死大鼠，取脑及血液，制作石蜡切片，光学显微镜下观察挫伤脑皮质的形态学变化。取脑皮质制成悬液，置721分光光度计，检测脑皮质中SOD、MDA、GSHPX和Ca2+含量，试剂盒均由南京建成生物工程研究所生产。数据经统计学处理（t检验）。 　　2 结果 　　伤后