the relative importance of topography and rgd ligand density for endothelial cell adhesion地形的相对重要性和内皮细胞粘附rgd配体密度.pdfVIP

The Relative Importance of Topography and RGD Ligand Density for Endothelial Cell Adhesion 1,2 2 ¨ 1,2 2 1 Guillaume Le Saux , Astrid Magenau , Till Bocking , Katharina Gaus *, J. Justin Gooding * 1 School of Chemistry, University of New South Wales, Sydney, New South Wales, Australia, 2 Centre for Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia Abstract The morphology and function of endothelial cells depends on the physical and chemical characteristics of the extracellular environment. Here, we designed silicon surfaces on which topographical features and surface densities of the integrin binding peptide arginine-glycine-aspartic acid (RGD) could be independently controlled. We used these surfaces to investigate the relative importance of the surface chemistry of ligand presentation versus surface topography in endothelial cell adhesion. We compared cell adhesion, spreading and migration on surfaces with nano- to micro-scaled pyramids and average densities of 6 6102 11 2 –6 610 RGD/mm . We found that fewer cells adhered onto rough than flat surfaces and that the optimal average RGD density for cell adhesion was 6 6105 RGD/mm2 on flat surfaces and substrata with nano-scaled roughness. Only on surfaces with micro-scaled pyramids did the topography hinder cell migration and a lower average RGD density was optimal for adhesion. In contrast, cell spreading was greatest on surfaces with 6 6108 RGD/mm2 irrespectively of presence of feature and their size. In summary, our data suggest that the