A_rapid_method_of_total_lipid_extraction_and_purification英文资料.pdf

Canadian Journal of Biochemistry and Physiology Issued by THENATIONAL RESEARCH COUNCILOF CANADA VOI,UME 37 AUGUST 1959 NUMBER 8 A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION1 Abstract Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues. Introduction In the course of investigations concerning the deterioration of lipids in frozen fish, the need arose for an efficient and rapid method of total lipid extraction and purification. Furthermore, due to the highly unsaturated nature of fish lipids, the method had to involve only mild treatment so as to minimize oxidative decomposition and the production of artifacts. Several existing methods were considered but none was entirely