《纤维素酶的基因克隆及表达研究进展.》-毕业论文.docVIP

PAGE 1 PAGE 2 - - 湖 北 大 学 毕 业 论 文 设 计 题 目 纤维素酶的基因克隆及表达研究进展 专业年级 生物工程 学生姓名 学 号 指导老师 年 12 月 5 日 目 录 1.前言4 1.1 纤维素酶的组成4 1.2 纤维素酶的分类与分布5 1.3 纤维素酶降解纤维素的机理研究6 1.4 纤维素酶的分子结构7 1.5 纤维素酶在畜禽生产中的应用9 1.5.1 在牛日粮中的应用9 1.5.2 在鸡日粮中的应用9 1.5.3 在猪日粮中的应用10 2．纤维素酶的研究现状及展望10 3. 纤维素酶的基因克隆与表达10 3.1 纤维素酶的基因克隆方法10 3.2 纤维素酶活力的测定10 3.3 实验材料试剂12 3.4 克隆基因13 3.5 重组质粒的构建13 3.6 完整基因序列的克隆和分析13 4. 实验分析及讨论14 参考文献15 Cloning and Expression of Cellulase Research Cellulose is an important enzyme product, is a complex enzyme, mainly by the exo β-glucanase, endo-β-glucanase and β-glucosidase enzyme component, there is a high energy Xylanase activity. Since cellulose in feed, alcohol, textile and food industries have great market potential, has been optimistic about the industry at home and abroad, will be following the glucoamylase, amylase and protease enzymes after the fourth-largest industrial species, even in China Entirely possible to become the first major enzyme species, the cellulase enzyme preparation industry is a new growth point. Cellulose is composed of many highly synergistic enzymes composed of catalytic reactions based on their different functions can be divided into endoglucanase (1,4-β-D-glucan glucanohydrolase or endo-1 ,4-β -D-glucanase, EC, the C1 enzyme), from fungi referred to as EG, from bacteria called Cen, exo-glucanase (1,4-β-D-glucan cellobilhydrolase or exo-1,4-β-D-glucannase, EC.1), from the fungus referred to as CBH, from bacteria called Cex) and β-glucanase (β-1, 4 - glucosidase, EC.3.2 .1.21) referred to as BG. Cellulose molecule is a globular catalytic (Catalytic Domain, CD) or through an hydroxy amino acid proline-rich connecting bridge (Linker) and no catalytic domain of cellulose synthesis (carbohydrate-binding modules, CBM) three Parts. Only a small