金银花提取工艺的分析-化学工艺专业论文.docxVIP

中文摘要本文对金银花药材分别采用煎煮法、乙醇回流提取法与超声波辅助提取法进 中文摘要 本文对金银花药材分别采用煎煮法、乙醇回流提取法与超声波辅助提取法进 行了实验研究，应用高效液相色谱质谱联用一紫外分光检测器对绿原酸含量进行 定量分析；计算出绿原酸的提取率，并考察了每种提取方法的工艺参数对提取效 果的影响。 正交试验设计结果表明，在煎煮法中影响出膏率和绿原酸提取率的主要因素 是提取液pH值，提取温度，预浸泡，溶媒用量和提取时间。乙醇回流法中的主 要影响因素是乙醇浓度，提取液pH值，溶媒用量和提取时间。由单因素实验结 果可知，在酸性条件下有利于绿原酸的提取。在本实验范围内，煎煮法中的最佳 提取温度为70℃，回流法中的溶媒(乙醇水溶液)的乙醇体积分数最佳值为70％。 研究表明，超声场对中药提取过程有强化作用，超声波空化效应对提取绿原 酸有较大影响。从正交试验设计结果得到，超声功率和乙醇浓度是影响出膏率和 绿原酸提取率的主要因素。在本实验范围内，超声波辅助提取在酸性条件和超声 功率为100W·g。时，可达到最佳效果。 对比三种实验方法可以看出，超声波辅助提取工艺中绿原酸提取率由煎煮法 和乙醇回流提取法的35．46 mg·g-1和41．29 mg·g-1上升到59．23 mg·g-1，提取时间 由传统工艺的2．5h缩短到lh。因此超声波辅助提取是一种较佳的提取工艺。在 本实验范国内，超声波辅助提取工艺的最佳提取条件是：药材粉碎20目，未预 浸泡，超声功率为100W·g～，乙醇浓度为70％，溶液pH值调至4．0，溶媒用量 16倍于药材的质量，提取2次，每次30min。 本文的研究结果为绿原酸的提取和生产中的应用提供了理论基础。 关键词：绿原酸超声波辅助提取金银花超声功率正交试验 AB AB STRACT Extracting techniques of honeysuckle were experimentally studied by using water-extraction，alcoh01．extraction and ultrasonic-assisted extraction 00AE)．The chlorogenic acid was extracted from honeysuckle and its content was analyzed with the LC。MS／MS method．With the gained results the yield was calculated and the technical parameters which could influence the extracting effects were discussed． The factors of extracting solution’s pH，extracting temperature，pre·soakage， alcohol dosage，extracting time in water-extraction method and the factors of alcohol concentration，extracting solution’S pH，alcohol dosage，extracting time in alcoh01．extraction were the main effect factors。to the honeysuckle paste and chlorogenic acid yield according to the orthogonal test．The acidic condition which was helpful to the extracting of chlorogenic acid was gained from the univariate experiments．Under the conditions of experiments，the optimal temperature Was 70"C in water-extraction method and alcohol dosage was 70％in alcohol concentration． According to the research，ultrasonic could intensify the Chinese traditional medicine’S extraction and ultrasonic cavitate effect had great influence on the chlorogenic acid extraction．The ultrasonic power an