L-半胱氨酸和胱胺共自组装膜活体检测抗坏血酸.pdfVIP

第25 卷 第3 期 电化学 Vol. 25 No. 3 2019 年6 月 JOURNAL OF ELECTROCHEMISTRY Jun. 2019 DOI: 10.13208/j.electrochem.181045 Artical ID:1006-3471(2019)03-0400-09 Citethis: J. Electrochem. 2019, 25(3): 400-408 Http:// Cysteineand Cystamine Co鄄Self鄄AssembledMonolayersfor in VivoDetectionofAscorbicAcid ZHANG Yue, FENG Tao-tao, JI Wen-liang, ZHANG Mei-ning* 渊Department of Chemistry,Renmin Universityof China,Beijing 100872, China冤 Abstract: Self-assembled monolayers (SAMs), which form highly ordered monolayers on the electrode surface through the gold-suffer bond, have attracted much attention in recent years. This stable layer not only can regulate the wettable properties of sur- face, but also can act as a promoter towards redox-active molecules. Here, we developed a simple and effective method to construct cysteine and cystamine co-self-assembled monolayer on gold microelectrode for invivodetection of ascorbic acid (AA). The molar ratio at 1:1 of mixed monolayer has been found the optimum to enhance the electron-transfer kinetics of AA oxidation at low poten- tial (ca. 0.10 V), meanwhile, it could resist the non-specific adsorption of protein at electrode surface. The application of the co-self-assembled monolayer is preliminarily demonstrated for invivodetection and the basal level of striatum AA was determined -1 to be 257 依30 滋mol 窑L (n= 3). This study offers a general and effective approach for invivoelectrochemistry with high reliability and simplified procedures. Keywords: cysteine and cystamine; co-self-assembled monolayer; ascorbic acid; anti-adsorpt