Principles of Fluorescence Spectroscopy 厦门大学研究 荧光光谱的原理厦门大学研究.ppt

Principles of Fluorescence Spectroscopy Chemistry Department XMU Chapter Three Fluorophores 3.1 Organic Fluorescent Molecular 3.2 Metal-ligand compounds 3.3 Probes 3.4 Reference Probes 3.3.1 Protein probes 3.3.2 Membrane probes 3.3.3 DNA probes 3.3.4 pH probes 3.3.5 Ion probes 3.3.6 Potentialmetric probes Reference Molecular probes , Handbook of Fluorescent Probes and Research Chemicals Molecular Probes Molecular probes homepage Protein fluorescence Protein-labeling Probes Protein-labeling Probes photo TRITC Tetramethylrhodamine 5- (and 6) isothiocyanate RG Oregon Green 514 carboxylic acid Staining 6-acryloyl-2-dimethylaminonaphthalene (acrylodan)6 - 烯丙酰- 2 - 二甲胺基萘（highly sensitive to polarity) Texas Red Comparison of protein labeling Reaction Noncovalent protein-labeling probe Membrane probes Ion channel Ion channel Partitioning probe Membrane probes Membrane probes Membrane probes Membrane probes Partition DNA probes DNA staining DNA staining DNA staining DNA staining DNA staining DNA and RNA determination DNA and RNA determination pH indicator Ionization equilibria of fluorescein Ca2+ indicator fluo-3 Mg2+ indicator Magnesium Green Zn2+ indicator Cd2+ indicator Na+ indicator SBFI K+ indicator PBFI Comparison Inorganic phosphate indicator Cl- indicator Potentiometric probes 　电位探针 Potentiometric probes Potentiometric probes Potentiometric probes Potentiometric probes Potentiometric probes Potential-dependent staining cell images Hoechst 33258 (bis-benzimide) Absorption and fluorescence emission spectra of Hoechst 33258 bound to DNA. Normal and camptothecin-treated bovine pulmonary artery endothelial cells (BPAEC) were stained with C4-BODIPY 500/510 C9 (B-3824 and Hoechst 33258 (H-1398, H-3569). BODIPY and Hoechst fluorescence were recorded using longpass filters appropriate for fluorescein and DAPI, respectively. An overlay of the images indicates that a red shift in