辐射诱导性自杀基因对胰腺癌体内治疗效果的实验观察.doc

辐射诱导性自杀基因对胰腺癌体内治疗效果的实验观察 目录 TOC \o "1-9" \h \z \u 目录 1 正文 1 文1：辐射诱导性自杀基因对胰腺癌体内治疗效果的实验观察 1 1 材料和方法 3 2 结果 4 3 讨论 5 文2：抗菌纳米材料对病原菌抗菌效果的实验观察 7 1 材料与方法 8 2 结果 9 2．1 抗菌剂对病原菌最高杀菌浓度的测定结果 见表1。 9 3 讨论 9 参考文摘引言： 10 原创性声明（模板） 12 文章致谢（模板） 12 正文 辐射诱导性自杀基因对胰腺癌体内治疗效果的实验观察 文1：辐射诱导性自杀基因对胰腺癌体内治疗效果的实验观察 The therapeutical effect of radio-inducible suicide gene on pancreatic cancer cell in vivo Abstract Objective The nude mouse model of pancreatic cancer was established by implanting PC-3 cell subcutaneously into the animal， to observe the therapeutical effect of pAdEgr-1-TK with irradiation and prodrug GCV addition to the cancer cell. Methods 1×107 PC-3 cells together with ml phosphate buffer solution (PBS) were implanted subcutaneously into 24 nude mice， which were divided equally into 4 groups. By the time when the tumor grew to cm， the group PC-3/pAdE was injected with the mixture of PBS and pAdEgr-1， pAdEgr-1-TK was directly injected into the tumor of group PC-3/pAdET (non-irradiated) and group PC-3/pAdET (irradiated) qd for 3 days in the dosage of 1×109 pfu/ml× ml per mouse， meanwhile， GCV was injected into the abdominal cavity (25 mg/kg/day) for 14 days， when the mice would be irradiated by 60Co-γ in dosage 10Gy and once more 48 hou later. The volumes of the tumor were measured every 3 days (longer diameter × shorter diameter 2/2). At last， the tumo were taken out of the killed mice to observe the pathologic changes under light microscope. Results Tumor successfully grew in all of the mice without variance during the latent period. No variance existed (P=) before treatment (irradiation and GCV). After the treatment， the growth of tumor was very fast in the control group but markedly slowed down in the experimental group， while the volume and weight of tumor of group PC-3/pAdET were apparently less than that of the other three. All above-mentioned indicated that TK gene promoted by Egr-1 gene was activated to tracript by γ-rays， and TK sui