贝那普利氨氯地平卡维地洛对主动脉内皮细胞单层脂类通透性的影响临床医学.doc

贝那普利氨氯地平卡维地洛对主动脉内皮细胞单层脂类通透性的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：贝那普利氨氯地平卡维地洛对主动脉内皮细胞单层脂类通透性的影响临床医学 2 1 材料和方法 3 121 大鼠主动脉内皮细胞分离和培养 3 122 内皮细胞单层通透性测定 4 13 统计学方法 5 2 结果 5 21 药物对EC单层脂类通透性的检测 5 22 药物对EC单层水的通透性的影响 5 23 药物对EC单层蛋白清除率的影响 5 3 讨论 5 文2：氨氯地平对原发性高血压患者主动脉根部顺应性的影响 7 1 对象和方法 8 2 结果 10 3 讨论 11 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 15 正文 贝那普利氨氯地平卡维地洛对主动脉内皮细胞单层脂类通透性的影响临床医学 文1：贝那普利氨氯地平卡维地洛对主动脉内皮细胞单层脂类通透性的影响临床医学 Abstract： Objective To investigate the effects of angiotein converting enzyme inhibitor benazepril, calcium channel blocker amlodipine besylate andβadrenoceptor agonist carvedilol on the permeability of rat aortic endothelial cell (AEC) The method of trauma-free isolation was adopted in the culture of rat AECs. Rat AECs were seeded on the nitrocellulose microporous filte. After an eight days seeding, the monolaye could be used for measuring the permeability. Before being perfused, monolaye were treated with Benazepril, Amlodipine and Carvedilol for four hou successively. The prepared filte were mounted on the Boydon chambe and perfused with hyperlipemia containing fluorescein isothiocyanate (FITC)labeled albumin. The fluid filtering through the monolaye and the filter was collected and the albumin concentration was measured. At the same time, the concentratio of cholesterol, triglyceride (TG), highdeity lipoprotein (HDL) and lowdeity lipoprotein (LDL) in the collected fluid were also measure by Benazepril and Amlodipine decreased the permeability of the confluent AEC monolaye to water, cholesterol, TG, HDL and LDL significantly (P). Amlodipine had more significant effects than the former two drugs. Carvedilol had no obvious Benazepril and Amlodipine may decrease the infiltration of lipids and lipoprotei into the subendothelial space, and can be thus used to prevent and ameliorate atherosclerosis. Key words： endothelium; capillary permeability; lipoprotein; benazepril; amlodipine; carvedilol