用于再灌注损伤研究的两种肝移植模型的筛选.doc

用于再灌注损伤研究的两种肝移植模型的筛选 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：用于再灌注损伤研究的两种肝移植模型的筛选 1 1　材料与方法 2 2　结果 4 3　讨论 5 文2：不完全竞争模型的超边际分析 6 （一）什么是超边际分析 7 （二）用于超边际分析的不完全竞争模型 8 （四）对策 11 4．不承诺本国货币与美元等强势货币自由兑换。 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 13 正文 用于再灌注损伤研究的两种肝移植模型的筛选 文1：用于再灌注损伤研究的两种肝移植模型的筛选 【ABSTRACT】 Objective: To select a more suitable liver traplantation model for studying ischemic reperfusion injury. Methods: SD rats were randomly divided into three groups: sham operation group (S), orthotopic liver traplantation group (OLT) and analogic liver traplantation group (AT). The requirement for micro-surgical skills, survival rate and deterioration of histomorphology after operation among each group were observed. At the same time , the levels of total bilirubin (TB), alanine aminotraferase(ALT), glutamic oxalacetic traaminase(AST) and tumor necrosis factor-α(TNF-α) in the serum at 24 hou after operation were recorded. Results: The levels of TB, ALT,AST and TNF-αin AT group and OLT group were higher than those in S group. In AT group the pathological changes were milder and the levels of TB, ALT,AST,TNF-αwere lower in AT group (P)comparing with OLT, and AT group required micro-surgical skills and had higher survival rate(P). Conclusio: AT simplifies the procedure of OLT. It really reflects the pathophysiologic coue of ischemic reperfusion injury, therefore it can be chosen as an ideal model for studying ischemic reperfusion injury in liver traplantation. 【KEY WORDS】 Liver traplantation·Reperfusion injury·Reperfusion 缺血再灌注损伤存在于任何术式肝移植手术过程中，是造成移植肝无功能的重要原因之一。为了研究肝移植过程中缺血再灌注损伤，大多采用Kamada等[1-2]提出的“双袖套法” 创建大鼠肝移植模型。此模型手术操作难度大，存活率低且不能排除免疫排斥因素对术后供肝的影响。因此，选择一种研究缺血再灌注损伤适宜的肝移植模型很有必要。 1　材料与方法 试验动物　清洁级SD大鼠，雌雄不限，鼠龄8~10周，质量250～300 g(平均280 g)。江苏省实验动物中心提供，许可证号：SYXK(苏)2002-0045。 主要材料与仪器　酶联免疫吸附试验(ELISA)检测试剂盒(北京晶美生物工程有限公司)，阿托品注射液、乙醚(上海第一生化药业有限公司)，注射用低分子量肝素钠(江苏万邦生化医药股份有限公司)。JEM-2000EX型透射电镜(日本，Olympus)，PM10SP型光学显微镜(日本，Olympus)，MiVnt显微图象分析系统(上海，光学仪器厂)，SP-2102UV型紫外可见