关于健脾化瘀清热药对大鼠胃溃疡愈合质量及其表皮生长因子受体mRNA表达的影响.doc

关于健脾化瘀清热药对大鼠胃溃疡愈合质量及其表皮生长因子受体mRNA表达的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：关于健脾化瘀清热药对大鼠胃溃疡愈合质量及其表皮生长因子受体mRNA表达的影响 1 文2：携带HGF基因的减毒沙门氏菌对大鼠胃溃疡愈合及CD34表达的影响 1 1材料和方法 3 参考文摘引言： 7 原创性声明（模板） 8 文章致谢（模板） 9 正文 关于健脾化瘀清热药对大鼠胃溃疡愈合质量及其表皮生长因子受体mRNA表达的影响 文1：关于健脾化瘀清热药对大鼠胃溃疡愈合质量及其表皮生长因子受体mRNA表达的影响 文2：携带HGF基因的减毒沙门氏菌对大鼠胃溃疡愈合及CD34表达的影响 【Abstract】 AIM: To investigate the effect of the attenuated Salmonella typhimurium (St) with HGF gene on acetic acidinduced rat gastric ulcer and the expressio of the healing of CD34 in the ulcer. METHODS： Seventyseven acetic acidinduced gastric ulcer rat models were established, and 5 rats were randomly selected out to observe the distribution of green fluorescent protein (GFP) in the gastric tissue, then the remaining rats were randomly divided into 3 groups (n=24): StHGF treatment group, St treatment group and NaHCO3 control group. Rats of each group were respectively given StHGF, StGFP, St and 1×105 mg/L NaHCO3 every other day orally. Histopathological examination of the ulcer tissue was performed by HE staining and the expressio of CD34 were detected by immunohistochemical method with SABC kit on day 7, 14 and 21 after administration. RESULTS: On day 7, gastric mucosa disappeared in ulcer site, a great of necrotic tissue was found, and gastric epithelium crawling was not obvious. On day 14 and 21, epithelium proliferated more quickly in StHGF group than in other 2 groups (P). The expression of CD34 protein in StHGF group was significantly higher than that in the other 2 groups (P). CONCLUSION: The attenuated Salmonella typhimurium carrying HGF gene could promote gastric mucosa repair and new vascular formation, thus accelerating the healing of ulce in experimental rats. 【Keywords】 vaccines, attenuated; salmonella typhimurium; stomach ulcer; healing; hepatocyte growth factor; CD34 0引言 减毒鼠伤寒沙门氏菌(attenuated Salmonella typhimurium, St)是一种良好的口服DNA载体. 研究发现, 在体内, St可以将编码外源基因的真核质粒递呈入抗原递呈细胞(如巨噬细胞、树突状细胞等), 并在其中表达及释放［1］. 肝细胞生长因子(he