姜黄素对小鼠心肌梗死后心室重构的保护作用及机制研究.docxVIP

姜黄素对小鼠心肌梗死后心室重构的保护作用及机制研究 摘要 目的：研究姜黄素对小鼠心肌梗死后心室重构的保护作用及机制。 方法：40只小鼠随机分为对照组、梗死组、姜黄素10mg/kg组及姜黄素30mg/kg组，采用冠状动脉结扎法制备心肌梗死模型，分别于梗死后1、4、8周处死小鼠，采用心脏重量指数、心脏病理学检查、超声心动图、分子生物学技术等手段检测心室重构指标和炎症指标。 结果：姜黄素对小鼠心肌梗死后心室重构有保护作用，表现出显著降低心室质量指数、降低心肌纤维化、降低心肌细胞凋亡率、降低肺部炎症细胞浸润等效应。此外，姜黄素还能明显下调TNF-α、IL-1β、IL-6等炎症因子的表达水平。 结论：姜黄素通过抑制炎症反应、减轻心肌纤维化、促进心肌细胞存活等多方面的作用机制，可以显著减轻心肌梗死后的心室重构，提高心肌梗死后的心肌结构和功能。因此，姜黄素可能是心肌梗死后心室重构的保护剂，有望成为心脏保健和防治心血管疾病的新型药物。 关键词：姜黄素；心肌梗死；心室重构；炎症反应；小鼠 Abstract Objective: To investigate the protective effect and mechanism of curcumin on ventricular remodeling after myocardial infarction in mice. Methods: Forty mice were randomly divided into control group, infarction group, curcumin 10mg / kg group and curcumin 30mg / kg group. The myocardial infarction model was prepared by coronary artery ligation method. The mice were sacrificed at 1, 4, and 8 weeks after infarction. The myocardial remodeling indicators and inflammatory indicators were measured by heart weight index, pathological examination of the heart, echocardiogram, molecular biology techniques, etc. Results: Curcumin had a protective effect on ventricular remodeling after myocardial infarction in mice, showing significant effects in reducing ventricular mass index, reducing myocardial fibrosis, reducing myocardial cell apoptosis rate, reducing pulmonary inflammatory cell infiltration, etc. In addition, curcumin can significantly down-regulate the expression levels of inflammatory factors such as TNF-α, IL-1β, IL-6. Conclusion: Curcumin can significantly reduce ventricular remodeling after myocardial infarction and improve myocardial structure and function after myocardial infarction by inhibiting inflammatory reactions, reducing myocardial fibrosis, and promoting myocardial cell survival. Therefore, curcumin may be a protective agent for ventricular remodeling after myocardial infarction and may become a new type of drug for cardiovascular disease prevention and treatment. Keywords: curcumin; myocardial infarction; ventri