等离子体聚合胺基功能薄膜及其细胞吸附性研究.docVIP

等离子体聚合胺基功能薄膜及其细胞吸附性研究 谢芬艳1，胡文娟1，翁静2，陈强*1 1-北京印刷学院印刷包装北京市重点实验室，北京大兴，102600 2-首都医科大学基础医学部，北京右安门，100069 摘要：本文采用射频等离子体（13.56MH）, 以丙烯胺（allylamine）为聚合单体,氩气为辅助气体,含成含胺基的生物功能薄膜。利用接触角测定仪（WCA），傅立叶变换红外光谱(FTIR)，X-射线光电子能谱(XPS)，原子力显微镜(AFM)对功能薄膜的结构，成分以及形貌进行测试表征。同时也通过体外细胞培养的方式研究了胺基生物功能薄膜对细胞黏附以及细胞生长的作用，并在倒置显微镜下观察细胞的黏附以及生长情况。研究了不同等离子体参数如：功率，工作气压，放电模式，占空比等对聚合功能薄膜表面结构成分、功能团含量以及生物性能等的影响。结论是：RF等离子聚合的胺基功能薄膜具有良好的生物相容性，能较好地促进细胞在其表面的黏附以及生长。 关键词：胺基功能薄膜；等离子体聚合；细胞吸附 Properties of amine-containing functional films by plasma polymerization Fenyan Xie1, Wenjuan Hu1, Jing Weng2, Qiang Chen*1 1- Laboratory of Plasma Physics and Materials, Beijing Institute of Graphic Communication, Beijing, 102600 2- Capital Medical University，Beijing , China，100069 Abstract：Using allylamine monomer, amine-containing functional films were prepared by plasma polymerization. The surface composition and microstructure of amine-containing functional films were characterized by water contact angle measure(WCA), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), and atomic force microscopy (AFM) measurements. In vitro cell culture cells adsorption and growth were also carried out in this paper, which the cell adsorption and morphology were acquired with a phase contrast microscope. The influence of plasma parameters，such as plasma power, discharge modes, explosive times on densities of amine were investigated in detail. And the functional films with a high concentration of amine promoting cell adsorption are also investigated in this paper. The conclusions show that RF plasma can polymerize excellent amine-containing functional films, which can effectively promote the cells adsorption and growth. Key words：amine-containing functional films；plasma polymerization；cell adhesive 1、引言 近几十年来，合成医用高分子材料在人工脏器、硬组织、植入物以及临床医用方面的应用日益广泛。但是，实践中常常发现，许多高分子材料在与生物体相接触时，二者会在界面处发生一系列复杂的相互作用，导致凝血反应和血栓的形成以及对生物体组织产生毒性作用[1]，从而使合成高分子材料在生物医学上的应用受到极大限制。 等离子体聚合是一种较好的材料改性以及薄膜沉积的手段，它能够在材料表面制备出化学惰性的涂层而不破坏和改变材料本身的结构和性