聚乙二醇接枝壳聚糖聚离子胶束的制备与体外表征研究_杨可伟.pdf

Chinese Journal ofNew Drugs 2007, Vol. 16No. 13 2007 16 13 , , , ( 北京大学药学院药剂学系, 北京 100083) [] : 制备载有甘草酸二铵(DG)的聚乙二醇接枝壳聚糖(mPEG-CS)聚离子胶束,考察胶束的 化性 质及体外释放机制: 通过壳聚糖烷基化合成mPEG-CS, 以三聚磷酸钠为引发剂, 利用静电相互作用制备载有 DG的mPEG-CS聚离子胶束考察胶束的形态粒径和 zeta电位用透析袋法研究载药胶束在不同介质中的释放 动力学: 制得胶束的平均粒径为( 38. 2? 019) nm; 包封率为( 97. 6? 0. 7)%; 胶束的稳定性良好; 胶束的释放 与释放介质的种类p 值和浓度有关: mPEG-CS是一种良好的制备聚离子胶束的高分子材料, 聚离子胶束 是离子型药物的良好载体 [] 聚离子胶束; 壳聚糖; 甘草酸二铵; 体外释放 [] R943. 42; R97515 [] A [] 1003- 3734( 2007) 13- 1030- 05 D iammonium glycyrrhizinate loaded methoxy PEG-grafted-chitosan polyion complex m icelles: preparation and in vitro characterization YANG Ke-wei, LIXin-ru, YANG Zhuo-l,i LIU Yan (Department of Pharmaceutics, School of PharmaceuticalSciences, Peking University, Beij ing 100083, China) [A stract] O jectives: To prepare and characterize the diammonium glycyrrhizinate loadedme- thoxy poly( ethylene glycol)-grafted-chitosan polyion complex micelles(mPEG-chitosan PIC micelles). M ethods: mPEG-chitosan was synthesized by alkylation of chitosan w ith mPEG-aldehyde. mPEG-ch-i tosan PICmicelleswere prepared using sodium tripolyphosphate as an initiator via the electrostatic inter- action. Themicelles were observed under the transm ission electron microscopy(TEM ). M ean size and zeta potentialvalues of themicellesweremeasured using a zetasizer based on the dynam ic light scatter- ing. In vitro releaseof the diammonium glycyrrhizinate-loadedmPEG-chitosan PICmicelleswasmeasured using a dialysis method in various buffered solutions. Results: Diammonium glycyrrhizinate-loaded mPEG-chitosan PICm icelleswe