双分子荧光互补试验研究水稻nh1 家族蛋白之间相互作用 - 华北农学报.pdf

华北农学报 20 11, 2 6 ( 2) : 30-34 NH 1 1 2 3 3 3 4 白 薇, 孙海莲 , M awsh ng Ch rn , Randy Ruan , Pam Ronald , 樊明寿 ( 1. , 010018; 2. , 010031; 3. D partm nt of Plant Patho logy, Un iv rsity of Californ ia, Davis, CA 95616. U SA; 4. , 010018) : NPR1( Non- xpr ss r of pathog n sis r lat d g n s 1)( Salicilic acid, SA) ( Sys- t m ic aqu ir d r sistanc , SAR), NPR1 NH 1/OsNPR 1 ( NPR1 hom o- logu 1) ( NPR1) 4 NPR1 ( Paralog), NH 1 ( B mi ol cu lar Fluor sc nc Com pl m ntation, B iFC, or sp lit Y FP) NH 1 , NH2, NH 1NH 3NH 4 NH 5, , : ; NH 1; ; : S511 : A : 1000- 7091( 2011) 02- 0030- 05 Protein-P rotein In teraction BetweenM em bers of NH 1 Fam ily in B im olecu lar F luorescence Com p lem en tation A ssay 1 2 3 3 3 4 BA IW i, SUN H a-i lian , M aw sh ng Ch rn , Randy Ruan , Pam Ronald, FAN M ing-shou ( 1. Coll g of Lif Sci nc s, Inn rM ongoliaAgriculturalUniv rsity, Huhhot 010018, Ch ina; 2. Inn rM ongolia Grassland R s arch C nt r, Chin s Acad my of Sci nc s, Huhhot 010031, China; 3. D partm nt of Plant Pathology, Un iv rsity of California, Davis, CA 95616, USA ; 4. Coll g ofAgronomy, Inn rM ongoliaAgriculturalUniv rsity, Huhhot 010018, Ch ina ) Abstract: NPR 1(Non- xpr ss r of pathog n sis r lat d g n s 1) is th mast r r gulator of salicylic acid-m d ia- t d systm ic acqu ir d r sistanc . Ov r- xpr ssion ofA rab idopsisNPR1 and ric NH 1 (NPR1 homolog1 ) /OsNPR1 in ric r sults in nhanc d r sistanc . Th r ar four ric NPR 1-paralogs in th ric g nom , th y ar fam ily m mb rs of NH 1. Prot in-prot in int raction b tw n m mb rs of NH 1 family w r studi d