2种疾病患者尿液有机酸代谢谱主成分的确定及分析.docVIP

2种疾病患者尿液有机酸代谢谱主成分的确定及分析 马振菁 黄建明 吴珍琴 郑秀芬 林绥 邓思珊 刘洪旭 福建省医学科学研究院福建省医学测试重点实验室 福建省测试技术研究所有机分析室 福建省立医院神经内科 X 关注成功！ 加关注后您将方便地在 我的关注中得到本文献的被引频次变化的通知！ 新浪微博 腾讯微博 人人网 开心网 豆瓣网 网易微博 摘????要： 目的 采用主成分分析法分析重症肌无力、线粒体肌病和健康者尿中22种有机酸代谢产物的特征, 为临床筛查提供资料和依据。方法 通过获得主成分及相应的主成分方程, 解释主成分的理化意义。结果 前5个主成分累积贡献率达到86.89%, 被确定为主要成分;建立主成分函数表达式, 分析主成分与原始变量之间的关系;发现苯基饱和酸类可能是2种疾病的潜在生物标志物成分;马尿酸为2种疾病预警成分。结论 尿有机酸代谢谱主成分分析, 有助于疾病的生物标志物的发现和为辅助临床诊断提供科学依据。 关键词： 主成分分析; 线粒体肌病; 重症肌无力; 尿有机酸代谢谱; 作者简介：马振菁:mzjing963@163.com 收稿日期：2017-07-21 基金：省属公益类科研院所基本科研专项 (2014R1031-1) Principal component determination and analysis of urine organic acid metabolism spectrum of two diseases MA Zhen-jing HUANG Jian-ming WU Zhen-qin ZHENG Xiu-fen LIN Sui DENG Si-shan LIU Hong-xu Fujian Key Laboratory of Medical Measurement, Fujian Academy of Medical Sciences; Organic Analysis Room, Fujian Institute of Testing Technology; Dept. of Neurology, Fujian Provincial Hospital; Abstract： Objective Analysis of 22 kinds of organic acid metabolites in urine samples of patients with myasthenia gravis, mitochondrial myopathy and of healthy controls was performed so to provide data and basis for clinical screening. Methods The principal component and the corresponding principal component equation were obtained, the physical and chemical significance of the principal component was explained. Results The cumulative contribution rate of the first five principal components reached 86. 89%, was identified as the main component, then established the principal component function expression, and analyzed the relationship between the principal component and the original variable. It was found that the phenyl saturated acid might be a potential biomarker of the two diseases, and the hippuric acid was an early warning bio-marker of the two diseases. Conclusions Urine organic acid metabolic profile principal component analysis is helpful to find biomarker of disease and may support clinical diagnosis basis. Keyword： principal component analysis;