絮凝酵母吸附CrVI的机理-微生物学报.PDF

生 物 工 程 学 报 Chin J Biotech 2011, January 25; 27(1): 52−59 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2011 CJB, All rights reserved. 环境生物技术 絮凝酵母吸附Cr(VI) 的机理 陈丽杰，王志存，葛旭萌，白凤武 大连理工大学生命科学与技术学院，大连 116024 摘 要: 絮凝酵母SPSC01 为酿酒酵母Saccharomyces cerevisiae 和粟酒裂殖酵母Schizosaccharomyces pombe 的融合菌 株，用其吸附水溶液中的重金属 Cr(VI) ，可以大大降低生物吸附的固液分离成本。为了探讨 SPSC01 菌体絮凝蛋白对 Cr(VI) 还原吸附的影响，对 SPSC01 与其亲本菌株的吸附行为进行了比较。结果表明，SPSC01 和其具有絮凝性状的亲 本S. pombe 的Cr(VI) 去除速率基本同步，远优于无絮凝性状的亲本S. cerevisiae ；达到吸附平衡时，S. pombe 、SPSC01 和S. cerevisiae 对总Cr 去除率分别达68.8 ％、48.6 ％和37.5 ％；从而证明了絮凝有利于Cr(VI) 的还原、吸附，絮凝蛋 白在Cr(VI) 的还原吸附过程中起促进作用。通过化学屏蔽方法和傅立叶变换红外光谱 (FTIR) 分析，对 SPSC01 菌体表 面吸附Cr(VI) 的机理进行了研究，结果表明SPSC01 菌体表面吸附Cr(VI) 起主要作用的基团是氨基、羧基和酰胺基。 关键词: 絮凝酵母，生物吸附，Cr(VI) ，吸附机理 Mechanism of Cr(VI) biosorption by flocculating yeast Lijie Chen, Zhicun Wang, Xumeng Ge, and Fengwu Bai School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China Abstract: The flocculating yeast strain SPSC01 is a fusant strain of Saccharomyces cerevisiae and Schizosaccharomyces pombe. The use of SPSC01 to absorb Cr(VI) from Cr(VI) containing aqueous solution would greatly reduce the cost of post-adsorption separation, since the superior flocculating property of SPSC01 would allow easy separation of the Cr(VI)-biomass from the solution. In order to investigate the effects of flocculating proteins on Cr(VI) reduction and absorption by SPSC01, the absorption behaviors of SPSC01 and its parental strains were compared. The results showed that Cr(VI) removal rate of SPSC01 wa