核苷酸序列分析以及应用.ppt

Correspondence analysis of codon usage variation among L. lactis (乳糖醇) genes. Each gene is plotted using its gene name at its coordinate on the first two axes produced by the analysis. The putatively highly expressed genes lie towards the left extreme of axis 1. The ellipse indicates a group of genes that putatively encode integral membrane proteins(内在膜蛋白). Genes with encode regulatory genes are plotted in red, those genes encoding proteins that are involved in the metabolic pathways for amino acid production are plotted in blue, and those genes encoding glycolytic (糖分解的)enzymes are plotted in green Correlation between the Effective Number of Codons for 123 L. lactis genes and correspondence analysis axis 1. Factor map of the 59 L. lactis synonymous codons on the two principal axes produced by the analysis. Those codons which were identified as optimal are indicated by red, and rare codons are indicated by blue. * * 1.原核,简单的基因结构 2.真核 3.微生物，原核生物基因组 * * http://splicenest.molgen.mpg.de :人，果蝇，拟南芥 输入基因组序列Z83819 输入序列: AF166326 AF166327 选择性剪切（Alternative splicing）分析 核苷酸序列分析 Gene Structure 选择性剪切（Alternative splicing）分析 选择性剪接是调控基因表达的重要机制 了解不同物种、细胞、发育阶段、环境压力下基因的调控表达机制 分析方法： 查询选择性剪切相关的网站 多序列比对 查询选择性剪切相关的网站 核苷酸序列分析 Gene Structure 从已知基因的功能推测剪切机制 分析EST序列的选择性剪切 Seq1 ATGGGAAACTGGGTGGTTAACCACTGGTTTTCAGTTTTGTTTCTGGTTGTTTGGTTAGGGCTGAATGTTT TCCTGTTTGTGGATGCCTTCCTGAAATATGAGAAGGCCGACAAATACTACTACACAAGAAAAATCCTTGG GTCAACATTGGCCTGTGCCCGAGCGTCTGCTCTCTGCTTGAATTTTAACAGCACGCTGATCCTGCTTCCT GTGTGTCGCAATCTGCTGTCCTTCCTGAGGGGCACCTGCTCATTTTGCAGCCGCACACTGAGAAAGCAAT TGGATCACAACCTCACCTTCCACAAGCTGGTGGCCTATATGATCTGCCTACATACAGCTATTCACATCAT TGCACACCTGTTTAACTTTGACTGCTATAGCAGAAGCCGACAGGCCACAGATGGCTCCCTTGCCTCCATT CTCTCCAGCCTATCTCATGATGAGAAAAAGGGGGGTTCTTGGCTAAATCCCATCCAGTCCCGAAACACGA CAGTGGAGTATGTGACATTCACCAGCATTGCTGGTCTCACTGGAGTGATCATGACAATAGCCTTGATTCT CATGGTAACTTCAGCTACTGAGTTCATCCGGAGGAGTTATTTTGAAGTCTTCTGGTATACTCACCACCTT TTTATCTTCTATATCCTTGGCTTAGGGATTCACGGCATTGGTGGAATTGTCCGGGGTCAAACA