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17.9.27 (f) Water bath.—48–50°C.
AOAC Official Method 2000.06 (g) Water baths.—Circulating, thermostatically controlled,
Salmonella in Foods 43 ± 0.2°C, and 42 ± 0.2°C.
with a Low Microbial Load, Detection (h) Sterile spoons.—Or other appropriate instruments for trans-
First Action 2000 ferring food.
(i) Sterile glassware.—Culture dishes, 15 × 100 mm; glass or
(Applicable for the detection of Salmonella in foods with a low mi- plastic sterile pipets, 1 mL, with 0.01 mL graduations; and 5 and
crobial load.) 10 mL, with 0.1 mL graduations.
Caution: Potassium cyanide (KCN) broth is extremely poison- (j) Inoculating needle and inoculating loop .—Ca 3 mm id or
ous. Dehydrated selenite cystine (SC) broth is very 10µL, nichrome, platinum-iridium, chromel wire, or sterile plastic.
toxic if inhaled or swallowed. SC broth is very toxic if (k) Sterile test or culture tubes .—16 × 150 mm and
swallowed. 20 × 150 mm; serological tubes, 10 × 75 mm or 13 × 100 mm.
(l) Test or culture tube racks.
See Tables 2000.06A and B for the results of the inter- and (m) Vortex mixer.
intralaboratory studies supporting acceptance of the method.
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