缺血预处理加用川芎嗪抑制肠缺血再灌注性肝细胞凋亡.doc

缺血预处理加用川芎嗪抑制肠缺血再灌注性肝细胞凋亡 目录 TOC \o "1-9" \h \z \u 目录 1 正文 1 文1：缺血预处理加用川芎嗪抑制肠缺血再灌注性肝细胞凋亡 1 1 材料和方法 3 2 结果 4 3 讨论 5 文2：亚低温缺血预处理对大鼠肠缺血再灌注肾损伤的保护作用 7 1 材料与方法 8 2 结 果 9 3 讨 论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 缺血预处理加用川芎嗪抑制肠缺血再灌注性肝细胞凋亡 文1：缺血预处理加用川芎嗪抑制肠缺血再灌注性肝细胞凋亡 Abstract Objective To investigate the effects of ischemic preconditioning (IPC) plus tetramethylpyrazine on apoptosis of hepatic cell in intestinal ischemia reperfusion in rats and explore the possible mechanism. Methods SD rats were randomly divided into sham operated control group (group S)， intestinal ischemia reperfusion group (group IIR)， tetramethylpyrazine group (group LGT)， IPC group (group IPC) and IPC+tetramethylpyrazine group (group IPC+LGT). Expression of Bcl-2 and Bax protein in liver tissues was detected with immunohistochemistry， and apoptosis of cells in liver tissues was detected with terminal deoxynucleotidyl-traferase mediated dUTP nick end labeling (TUNEL). The activities of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver tissues were determined. Results The apoptosis index of liver cells in group IPC+LGT was lower than those in other groups except S group obviously， and the expression of Bcl-2 increased while the expression of Bax decreases which result in the raise of Bcl-2/Bax ratio in this group. At the same time， SOD activity rises up while MDA content declined in liver tissues in group IPC+LGT. Conclusion The combined use of tetramethylpyrazine and IPC shows obvious synergistic effects on inhibiting apoptosis in liver tissues after intestinal ischemia reperfusion. Key words ischemic preconditioning； ligustrazine； apotosis； reperfusion injury； liver； intestine 肠缺血再灌注损伤（intestine ischemical reperfusion，IIR）是外科常见的病理过程，可引起肠壁组织一系列病理生理改变，并引起远隔器官的次级损伤[1]。由于肝脏在机体的特殊地位和重要作用，所以肠缺血导致肝损伤越来越引起人们的重视，其机制至今尚未被完全阐明，目前认为与细胞凋亡有关。近年来 文献 报道显示，对于IIR后肠壁组织细胞凋亡的抑制有多种方法，包括缺血预处理(ischemic preconditioning，IPC)和各种化学预处理，但目前对IIR后肝细胞凋亡干预措施的研究少有报道。本