褪黑素对大鼠脊髓损伤后神经细胞凋亡的影响.doc

褪黑素对大鼠脊髓损伤后神经细胞凋亡的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：褪黑素对大鼠脊髓损伤后神经细胞凋亡的影响 1 1 材料与方法 2 2 结 果 4 3 讨 论 4 文2：褪黑素对大鼠脊髓损伤后iNOS表达的影响 5 1 材料与方法 6 2 结果 8 3 讨论 9 参考文摘引言： 10 原创性声明（模板） 12 文章致谢（模板） 12 正文 褪黑素对大鼠脊髓损伤后神经细胞凋亡的影响 文1：褪黑素对大鼠脊髓损伤后神经细胞凋亡的影响 Effects of Melatonin on Rat Neuro Apoptosis After Spinal Cord Injury Abstract：Objective To observe the effect of melatonin(MT) on rat neural cell apoptosis after experimental spinal cord injury(SCI) in order to study the protective effect on Sixtysix Wister rats were randomly divided into 3 groups：sham group(no spinal cord injury )，group A (SCI group) and group B(MT group).Sham group only cut vertebral plate without spinal cord SCI model was induced using the modified Allen technique in group A and rats were treated with MT(100 mg/kg) in group B and the dehydrated alcohol of equal volume in group A after and nerves function were measured at 8 h、24 h、48 h、72 h and 7 d following SCI in group A and B，while Sham group was at 48 Cell apoptosis rate was increased at 24 h，reached their peaks at 48 h and descended 72 h after injury in group apoptosis rate in group B was significantly decreased as compared with group A at three time points(P＜ or P＜)，and the functional recovery was better in group B than in group MT can inhabit rat neural cell apoptosis after has protective effect on acute SCI. Key words：melatonin；spinal cord injury；apoptosis 本实验旨在观察褪黑素(melatonin，MT)对大鼠脊髓损伤(spinal cord injury，SCI)后脊髓组织神经细胞凋亡的影响，探讨其对脊髓损伤的保护作用，现报道如下。 1 材料与方法 动物分组 健康成年Wister大鼠(苏州大学实验动物中心提供)66只，体重220～300 g，雌雄不限，随机分为三组：假手术组(Sham组)6只，单纯SCI组(A组)及MT治疗组(B组)各30只。A、B两组又按SCI后不同时间点(8、24、48、72 h和7 d)各分为5个亚组，每个时间点6只。 模型制备与处理 以10％水合氯醛(400 mg/kg体重)腹腔注射麻醉。严格无菌操作下取正中切口，切除T8～10椎板，暴露硬膜囊并保持其完整。Sham组仅切除椎板暴露硬膜，未损伤脊髓；A、B两组按照改良Allen法制作中度脊髓损伤模型。B组于SCI后30 min内腹腔注射MT溶液(100 mg/kg体重，购自晶美上海公司，瑞士Alexis公司生产)，按照褪黑素说明书要求，用无水乙醇对褪黑素进行稀释；A组于同一时间内给予等量无水乙醇。术后常规青霉素40万U肌肉注射，1次/d；人工排尿，2次/d。 检测指标及方法 A和B组分别于术后8、24、48、72 h和7 d随机各取6只，用4％水合氯醛麻醉，从原切口暴露脊髓，以损伤处为