生物分离用晶胶介质的成孔机理与性能.pdfVIP

生物分离用晶胶介质的成孔机理与性能 * 姚克俭 ，贠军贤，沈绍传，何兴娇，陈芳 (浙江工业大学化学工程与材料学院 绿色合成技术国家重点实验室培育基地， 杭州310032，中国) 摘 要：超大孔连续床晶胶层析是一种新型生物分离技术，其介质的制备是该技术的关键。本文结合课题组 最近开展的研究工作，对丙烯酰胺基聚合物晶胶和纳米粒内嵌晶胶等两种晶胶介质制备中基质骨架的形成、 超大孔隙的形成机理、影响因素、晶胶介质性能及分析方法等进行了探讨。结果说明，以结晶致孔法制备这 两种晶胶，都充分利用了溶剂结晶的排他性，二者的成孔机理相似。用扫描电镜分析、透射电镜析、渗透性 能测试、停留时间分布测试、吸附层析等方法可获得晶胶介质的性能参数。所得的晶胶介质孔隙尺寸数10 ～ -11 -13 2 -4 -3 -6 -8 2 -1 200微米，水相渗透率在10 ～10 m 范围，等板高度在5 ×10 ～2 ×10 m ，轴向混合系数10 ～10 m s ， 适于生物大分子物质的分离纯化。 关键词：连续床；生物分离；晶胶；机理；性能 Pore-formation Mechanisms and Characteristics of Supermacroporous Cryogels for Bioseparation YAO Ke-jian ，YUN Jun-xian ，SHEN Shao-chuan，HE Xing-jiao ，CHEN Fang (State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology ，College of Chemical Engineering and Materials Science ，Zhejiang University of Technology ，Hangzhou 310032 ，China) Abstract Supermacroporous cryogel monolith chromatography is suggested as an efficient separation method for bioseparation and the preparation of cryogel adsorbents is very important. In this work, the formation mechanisms of cryogel matrix and supermacropores, the effect factors, the cryogel characteristics as well as the corresponding measurement methods in the preparation of polyacrylamide-based cryogel and nanoparticle-embedded cryogel have been discussed and analyzed based on the experimental investigations carried out in our group recently. From the experimental results, the pore-formation mechanisms of these two typical cryogels are similar and the removing of monomers and dispersed nanoparticles out of the solvent crystals due to the solvent crystallization is available in both producing processes. The cryogel characteristics