Na加H加交换体1反义基因磁性纳米微粒对SGC7901胃癌细胞形态学的影响临床医学.doc

Na加H加交换体1反义基因磁性纳米微粒对SGC7901胃癌细胞形态学的影响临床医学 目录 TOC \o "1-9" \h \z \u 目录 1 正文 1 文1：Na加H加交换体1反义基因磁性纳米微粒对SGC7901胃癌细胞形态学的影响临床医学 1 1 材料与方法 3 2 结果 5 文2：大鼠全脑缺血再灌注损伤神经元Na加K加医学 8 1材料和方法 9 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 15 正文 Na加H加交换体1反义基因磁性纳米微粒对SGC7901胃癌细胞形态学的影响临床医学 文1：Na加H加交换体1反义基因磁性纳米微粒对SGC7901胃癌细胞形态学的影响临床医学 【Abstract】 Objective To study the effects of Na+H+ exchanger 1 (NHE1) antisee gene magnetic nanoparticies on the morphology of SCG7901 gastric caner Antisee NHE1 eukaryotic expression on vector was cotructed with recombinant DNA technique and trafected into SGC7901 gastric carcinoma cells by DCIONP. The dextran coated iron oxide nanoparticles (DCIONP) was synthesized with deposition. Then, NHE1 antisee gene magnetic nanoparticles were cotructed. We examined the morphological changes of the trafected and untrafected cells under light and electron DCIONP successfully trafected NHE1 antisee gene into SGC7901 gastric cancer cells. Under the light microscope, we found that trafected SGC7901 gastric cancer cells with NHE1 antisee gene were larger with abundant cytoplasm and comparatively same size of nuclei, and the ratio of nuclei to cytoplasm, mitotic count and heteromorphism decreased when they were compared with their parent cells. The electron microscope showed increased number of mitochondrion and size of the trafected SGC7901 gastric cancer cells with NHE1 antisee gene, expanded Golgi apparatus, rough endoplasmic reticulum and endoplasmic reticulum and strengthened heteropolarity. Conclusion The morphology and microstructure of SGC7901 gastric carcinoma cells change after the trafection of antisee NHE1 gene that can promote the differentiation of SGC7901 gastric carcinoma cells. 【Key words】 Na+H+ exchanger 1; Magnetic nanoparticles; Antisee gene therapy; Gastric cancer 传统的基因治疗方法大都缺乏靶向性，严重阻碍了基因治疗研究的快速 发展 。开发新的基因载体是增强基因治疗靶向性的关键问题。纳米技术的发展， 为解决基因转移载体问题提供了新的思路。体积微小、具有超顺磁性的氧化铁磁性纳米颗粒，有望成为理想的基因载体材料。胞膜离子交换蛋白Na+H+交换体1(Na+H+ exc