四种微量石蜡组织DNA提取方法的比较（临床医学资料）.docVIP

四种微量石蜡组织DNA提取方法的比较（临床医学资料） 文档信息 属性： F-01NHUN，doc格式，正文17706字。质优实惠，欢迎下载！ 适用： 作为医学资料、临床医学资料写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o "1-9" \h \z \u 目录 1 正文 1 文1：四种微量石蜡组织DNA提取方法的比较 2 1 材料与方法 3 2 结果 5 3 讨论 6 文2：四种血虚小鼠模型制备方法的比较 8 1 仪器和药品 8 2 方法 9 3 结果 10 三种造模方法的比较 10 4 讨论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 四种微量石蜡组织DNA提取方法的比较（临床医学资料） 文1：四种微量石蜡组织DNA提取方法的比较 ［ABSTRACT］ Objective: To explore the simple and effective method for DNA extraction from microamount formalin fixation and paraffin embedded tissues (FFPET). Methods: The DNA was extracted by four different methods: Chelex100 extraction, phenolchloroform purification, simple digestion method and water cooking method. The DNA fragment with 100500 bp length was amplified by PCR. It was analyzed by electrophoresis, and the procedure including PCR and analysis was repeated 1 year later. Results: For amplification of DNA less than 200 bp, the positive rates of Chelex100 group and simple digestion group were % and %, respectively, which were significantly effective than phenolchloroform purification group (%) (P<) and water cooking group (%) (P<). The amplication efficacy decreased as the length increase. The total positive rates of PCR were % in Chelex100 group, % in simple digestion group, % both in water cooking group and phenolchlorform group. And the positive rates in repeated assessment were %, %, % and %. Conclusio: Chelex100 extration method is easy and applicable in amplification analysis of DNA extracted from mircoamount paraffin embedded tissues; while simple digestion method and water cooking method are suitable to analysis of short length DNA fragment extracted from mircoamount paraffin embedded tissues. ［KEY WORDS］ Paraffin embedded tissue; DNA extraction; Chelex100 extraction 医院 病理科存在大量各种疾病甲醛固定石蜡包埋组织（formalin fixed and paraffin embedded tissues， FFPET），为分子病理研究提供了大量的信息来源。然而福尔马林固定后的蜡块包埋组织DNA降解严重［1］，档存FFPET组织切取量有限，传统酚氯仿提取DNA步骤繁杂，提取过程损失严重等都制约了石蜡组织在分子