《发育生物学》第12章Auxin Singaling and Transport.ppt

Auxin Singaling and Transport Team members: Huang Ji Lu Hongming Liu Tao Zhao Baodong The Importance of Auxin Embryogenesis all types of organogenesis root meristem maintenance vascular tissue differentiation hypocotyl and root elongation fruit ripening apical dominance growth responses to environmental stimu Methods to Observe Auxin Accumulation Auxin responsive promoter DR5（ARE—ARF—auxin） Immunolocalization of IAA Direct auxin measurements in tissue section Chemiosmotic hypothesis synthesis Transport of Auxin Circulation of PIN In Nucleus In Nucleus In Nucleus Others Feedback Regulation Summary Edification Systems Biology!!! (holism instead of reduction) Cooperation! Ectopic expression (in mammalian cell/lipid bilayers ) Indirect methods(DR5) Mutants! References Vieten A, Sauer M, Brewer PB, Friml J (2007) Molecular and cellular aspects of auxin-transport-mediated development. (Translated from eng) Trends Plant Sci 12(4):160-168 Badescu G Napier R (2006) Receptors for auxin: will it all end in TIRs? Trends in Plant Science 11(5):217-223 Parry G, et al. (2009) Complex regulation of the TIR1/AFB family of auxin receptors. Proceedings of the National Academy of Sciences 106(52):22540-22545 Pan J, et al. (2009) The E3 ubiquitin ligase SCFTIR1/AFB and membrane sterols play key roles in auxin regulation of endocytosis, recycling, and plasma membrane accumulation of the auxin efflux transporter PIN2 in Arabidopsis thaliana. (Translated from eng) Plant Cell 21(2):568-580 (in eng) Grunewald W Friml J (2010) The march of the PINs: developmental plasticity by dynamic polar targeting in plant cells. (Translated from English) Embo J 29(16):2700-2714 (in English) Weijers D Friml J (2009) SnapShot: Auxin Signaling and Transport. Cell 136(6):1172-1172.e1171. Vanneste S Friml J (2009) Auxin: A Trigger for Change in Plant Development. (Translated from English) Cell 136(6):1005-1016 (in English).. * * Independent Dependent: 1.IAM pat