《发育生物学》第9章Meristerms.ppt

WUS expression WUS encodes a homeodomain transcript factor and expresses in the lower part of central zone of SAM Why WUS can express in the important region? Who controls WUS exression specifically? In situ hybridization The SAM is very small WUS expression WUS encodes a homeodomain transcript factor and expresses in the lower part of central zone of SAM Why WUS can express in the important region? CLAVATA 1, 2, 3 Who controls WUS exression specifically? In situ hybridization CLAVATA 1, 2, 3 Wild type clavata1 The size of SAM in mutants of CLV are significantly increased. Wild type clavata1 Wild type clv3 Detect WUS expression in SAM of wild type and clv3 CLAVATA 1, 2, 3 Wild type clavata1 What would happen if CLV express too much? WT 35::CLV3 Wild type The phenotype of over-expressing CLV is similar to that of wus wus 35::CLV3 What is the relationship? WUS CLV What is the relationship? WUS CLV Where does CLV3 express? L1,L2: LCV3 L3: CLV1,LV2 Stem cells CLV1: Receptor-like protein kinase with LRRs and intracellular protein kinase domain CLV2: LLR Receptor-like protein CLV3 CLV1 WUS WUS WUS activities from early embryo STM WT stm1 WUS STM (STEMLESS) WUS WUS activities from early embryo till flower STM CLV AG WT stm1 Chapter 9 Contracture and development of apical meristem in shoot and root 1. SAM construction 2. SAM development3. RAM construction4. RAM development quiescent cent (qc) Construction comparative between SAM and RAM Anatomic structure of RAM 根冠 表皮 皮层 内皮层 维管束 不活动区 vascular bundle (v) columella (col) lateral root cap (lrc) epidermis (e) cortex (c) endodermis (en) quiescent cent (qc) Stem cell？ 根冠 表皮 皮层 内皮层 维管束 不活动区 vascular bundle (v) columella (col) lateral root cap (lrc) epidermis (e) cortex (c) endodermis (en) Stem cells in the RAM Chapter 9 Contracture and development of apical meristem in shoot and root 1. SAM construction 2. SAM development3. RAM construction4. RAM development quiescent cent (qc) Both SAM and RAM h