《发育生物学》第6章PCD.ppt

PCD in gametophytic development Tapetum Tapetum Stomium (裂缝） PCD in gametophytic development stomium When does the stomium formation? PCS1 encode an aspartic protease functions as an anti-cell-death component in reproduction and embryogenesis. PCS1 Wild type and transgenic flowers of Arabidopsis. PCS1 was fused with 35S promoter and the flower was treated with ethyle. Promotion cell survival 1,( PCS1) in Arabidopsis Ge X et al., 2005 WT 35:PCS1 Septum: 膈膜 Stomium:裂缝 What significance? Shedding resistant? Senescence and Programmed Cell Death in Plants 6.1 Senescence in plants 6.2 The mechanism of senescence in leaves 6.3 Programmed cell death in plants 6.4 The mechanism of PCD Keller(1972) and Wylli(1980) raised the concept of apoptosis in animal development. PCD is also called as apoptosis in animal science 细胞凋亡 Nematodes (线虫）Caenorhabditis elegans There are 1090 cells produced during embryogenesis of nematodes. The matured embryo consists of 959 cells and the other 131 cells died through the way of PCD. ? Cell death Cell death ced3- What function does ced3 has? Cell death ced9- ced3- What function does ced9 has? Cell death ced3- ced9- ced9- ced3- What is the relationship between ced3 and ced9? Cell death ced3- ced9- ced9- ced3- ced9 ced4 ced3 BCL2 Apaf1 caspase ced9 ced4 ced3 BCL2 Apaf1 caspase Genes for PCD in animals Organism Gene Protein Function n Nematodes ced-3 ICE 半胱氨酸蛋白水解酶 ced-4 钙结合蛋白 激活ICE ced-9 抑制蛋白 抑制ICE、钙结合蛋白 Mouse caspase ICE 半胱氨酸蛋白水解酶 BCL2 apoptosis inhibitor 抑制ICE Targets of ICE Nuclear proteins: polyADPR polymerase (DNA repair), Lamins (nuclear membrane proteins), …… Genes for PCD in animals Organism Gene Protein Function n Nematodes ced-3 ICE 半胱氨酸蛋白水解酶