《发育生物学》第4章Cell Communication.ppt

Tobacco mosaic virus movement protein fused to GFP, transiently expressed in a Nicotiana benthamiana leaf and imaged with confocal microscopy, 2007. MP-30, a viral movement protein, is thought to bind to the virus's own genome and shuttle it from infected cells to uninfected cells through plasmodesmata. MP-30 is able to increase the size exclusion limit from 700 Daltons to 9400 Daltons thereby aiding its movement through a plant Plasmodesmata are cell-cell channels interconnecting all the cells of the plant body into a huge syncytium, which makes plants ‘supracellular’ organisms. 4.1 Communication media 4.2 Communication through the memberane 4.3 Communication through plasmodesmata 4.4 Communication through long distance Chapter 4. Communication in plant Zea mays Vascular bundles Sieve element 筛管分子 Companion cell 筛胞 Vessel members 导管分子 Tracheids 管胞 Phloem 韧皮部 Xylem 木质部 管状分子 维管在植物的生长发育中起着“血管”作用 GA GA GA GA GA GA GA GA GA GA 维管在植物的生长发育中起着“血管”作用 Can a gene expressing products move through the vascular? A nice research in tomato 2.4 Position information in plant cells Cells at different position differentiate into different type of cells. Cells at different position get different information from neighbor cells Cells at different position get different information from long distance IAA RNA Me mutant in tomato: mouse ear 12.1 维管的结构和功能 PFP LeT6 PFP LeT6 PFP LeT6: A KN1 like gene encode a homeobox transcript 12.1 维管的结构和功能 PFP LeT6 PFP LeT6 PFP Me mutant: Two fused gene, homeobox transcript LeT6: A KN1 like gene encode a homeobox transcript Me mutant plant Wild type scion 米森科？ PFP LeT6 PFP LeT6 PFP Wild: Me: Fusion transcript RT-PCR Detect RNA in SAM 1Me 2 3Grafted 4Grafted In situ RT-PCR ? In situ RT-PCR In situ RT-PCR Fusion transcrip in Me mutant SAM LeT6 is present in the SAM of wild type plant In situ RT-PCR Fusion transcrip in Me mutant SAM Fusion transcrip is absent in the SAM of wild type plant LeT6 is present in the SAM of wild type pl