花椒体内外抗肿瘤作用及其机制的初步研究.docVIP

花椒体内外抗肿瘤作用及其机制的初步研究 目录 TOC \o "1-9" \h \z \u 目录 1 正文 1 文1：花椒体内外抗肿瘤作用及其机制的初步研究 1 1 材料 2 2 方法 3 3 结果 4 4 讨论 5 文2：雷公藤内酯醇体内外抗肿瘤作用 6 1材料与方法 6 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 花椒体内外抗肿瘤作用及其机制的初步研究 文1：花椒体内外抗肿瘤作用及其机制的初步研究 Abstract：ObjectiveTo study the anti-tumer effect and its immunological mechanism of the essential oil of zanthoxylum in vivo and in vitro. Methods Treating H22 cells at different time and with different concentration of the essential oil of zanthoxylum, MTS was used to assay the cell proliferation, flow cytometry was used to assay the cell apoptosis and cell cycles. The H22 BALB/C xenograft tumor modes were established. The anti-tumor effect of zanthoxylum was observed and tumor growth inhibitory rate (IR) was calculated. The content of serum IL-2 and IL-12 were examined by ELISA assay. ResultsTreating H22 cells 72h by 4mg/ml essential oil of zanthoxylum resulted in remarkable inhibition of the cell H22 cells 72h by 1mg/ml essential oil of zanthoxylum resulted in appearance of sub-G1 and increase G0/G1 phase cells and decrease S phase and G2/M phase cells. The essential oil of zanthoxylum inhibited the growth of tumor in tumor-bearing mice. However, essential oil of zanthoxylum could not increase the contents of murine serum IL-2 and IL-12. ConclusionThe essential oil of zanthoxylum inhibits proliferation and induces cell apoptosis of H22 cells, but it can not exert the anti-tumor efficiency through reinforcing the immune function. Key words： Zanthoxylum ; H22 cells; Apoptosis; Immune function 花椒属一种温热类中草药，辛,热,有小毒，归脾、肾两经,功能温中止痛,解毒杀虫，常用于 治疗 脾胃虚寒症。花椒还是深受民众喜爱的重要食用性香料，其资源在我国分布很广,安全性高。国内外研究报道花椒对消炎、镇痛、杀菌、抗病毒、抗寄生虫、抗真菌、抗氧化等均具有较强的作用［1～5］。随着对花椒提纯和药理作用的深入研究,发现花椒还具有抗肿瘤作用,但目前对花椒抗肿瘤的研究还不够深入,其体内的抗肿瘤作用研究则更少。为了认识花椒在体内外抗肿瘤作用的机制和特点,我们选用小鼠H22肝癌细胞为研究对象,观察了花椒在体内外对该细胞的作用，以期探讨花椒在体内外抗肿瘤作用及其可能的机制。 1 材料 成熟花椒,采于湖北宜都，经汪鋆植博士鉴定为Zanthoxylum bungeanum。H22腹水型小鼠肝癌细胞由本院保存，清洁级BALB/C小鼠由同济医科大学动物中心提供。MTS细胞增殖分析试剂由Promega公司提供。EPL CSXL流式细胞分析仪为Becman Coulter公司