肝细胞癌Tenascin蛋白和微血管密度检测及临床意义.doc

肝细胞癌Tenascin蛋白和微血管密度检测及临床意义 目录 TOC \o "1-9" \h \z \u 目录 1 正文 1 文1：肝细胞癌Tenascin蛋白和微血管密度检测及临床意义 1 1　资料与方法 2 2　结果 4 3　讨论 5 文2：老年非小细胞肺癌XIAP蛋白的表达及临床意义 6 1 资料与方法 6 2 结 果 7 3 讨 论 7 参考文摘引言： 8 原创性声明（模板） 9 文章致谢（模板） 10 正文 肝细胞癌Tenascin蛋白和微血管密度检测及临床意义 文1：肝细胞癌Tenascin蛋白和微血管密度检测及临床意义 【ABSTRACT】 Objective: To study the expression of tenascin in hepatocellular carcinoma(HCC)and to investigate the roles of tenascin expression in microvessel angiogenesis, invasion, metastasis and prognosis of HCC. Methods: Formalin fixed, paraffin wax embedded specime from 63 HCC patients were stained with an anti-tenascin monoclonal antibody, and the measurements were also done for paracancerous specime from the same patients and specime from 15 patients with liver cirrhosis and 10 normal liver. The correlation was analyzed between expression of tenascin and clinicopathological features as well as microvessel angiogenesis. Results: Tenascin immunoreactivity was observed in % samples of HCC, % of paracarcinoma, % of liver cirrhosis and negative of normal liver samples. Positivity and inteity of tenascin expressio in samples of HCC and paracarcinoma were higher than those in samples of cirrhotic and normal liver. Tenascin expression was correlated with Edmondson-Steiner grades, tumour numbe, capsule invasion, tumour metastasis and microvessel angiogenesis. Conclusion: The up-regulation of tenascin expression may play an important role in invasion and metastasis of HCC. 【KEY WORDS】 Carcinoma, hepatocellular·Tenascin·Microvascular deity 原发性肝癌是常见的恶性肿瘤之一，具有恶性度高、侵袭性强、切除后易复发和转移及预后差等特点，即使是小肝癌其5年肿瘤复发率高达50%。本研究采用免疫组织化学方法测定肝细胞癌(hepatocellular carcinoma，HCC)组织细胞外基质Tenascin(TN)及肿瘤微血管密度(microvascular deity，MVD)表达，探讨肝癌细胞外基质改变与临床病理因素的关系及对预后的影响。 1　资料与方法 标本来源与分组　收集济南军区总 医院 2000年1月—2004年12月经手术切除、病理组织学确诊的HCC标本63例，其中男56例，女7例;年龄24～72 岁，平均年龄(±)岁。病理分级(Edmondson-Steiner分级)：Ⅰ～Ⅱ级25 例，Ⅲ～Ⅳ级38例;TNM分期(UICC/AJCC第6版，2002年)：Ⅰ～Ⅱ期41例、Ⅲ～Ⅳ期22例;肿瘤直径～ cm，平均 cm;肿瘤包膜完整者28例，有包膜侵犯者35例;有转移者(包括淋巴结转移、肝外转移、胆管或门