用HPLC 柱后衍生法分析烟草中氨基甲酸酯类农药残留.pdf

2012-06 ，33 （3 ） Chinese Tobacco Science 97 HPLC * 650223 8 /NH -SPE FLD 2 8 0.11.0 mg/L 0.99510.99990.050.10.5 mg/kg 80.8%110.6%84.4%109.7%81.6%112.9%3.2%10.8%3.8%11.8%3.7% 8.5%0.0080.01 mg/kg S572.08 S481.8 1007-5119201203-0097-05 DOI 10.3969/j.issn.1007-5119.2012.03.020 Determination of Carbamates Residues in Tobacco by HPLC with Post-column Derivatization * SHAO Jinliang, WANG Luxiang, LIU Hongcheng , LAN Shanshan, MEI Wenquan, ZOU Yanhong, YANG Fang (Institute of Agriculture Quality Standards & Testing Technique, Yunnan Academy of Agricultural Science, Kunming 650223, China) Abstract: Residue analysis of 8 carbamate pesticides in tobacco by reversed-phase high performance liquid chromatography was assessed. Residues were first extracted with acetonitrile and water, filtrated with NH -SPE columns, and then determined by 2 post-column derivatization with fluorescence detector. The proposed methods showed a good linearity between analytical signal in the range of 0.1-1.0 mg/L with linear correlation coefficients of 0.9951-0.9999 and limits of detection of 0.008-0.01 mg/kg. The average recoveries obtained from samples spiked with standards at concentration level 0.05, 0.1 and 0.5 mg/kg were in the range of 80.8%-110.6%, 84.4%-109.7%81.6%-112.9%, respectively, with relative standard deviations of 3.2%-10.8%, 3.8%-11.8% 3.7%-8.5%. Asatisfactory result was obtained in the simultaneous determination of carbamate pesticide residues in tobacco. Keywords: high performance liquid chromatography; post-column d